LIPOSOME PREPARATION
 

OBJECTIVE:

Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation method.
 

REAGENTS:

Dioleoyl Phosphatidyl Choline

Buffer of choice / distilled water
 

METHODS:

    1. Dry 0.5 mmole of dioleoyl phosphatidyl choline under nitrogen in a disposable glass tube.
    2. Evacuate in dessicator under vacuum for 30 minutes.
    3. Add buffer / dH20 to required volume and scrape the sides of the glass tube to dislodge the lipid.
    4. Add protein at 1 mg/ul of lipid used.
    5. Vortex for 30 seconds. Sonicate twice in a bath sonicator at 7 degree for 15 sec.
This makes multilamellar vesicles that become small unilamellar vesicles (SUV) with prolonged sonication time. To make large unilamellar vesicles, use the extruder.


Updated 01 December 2000